Transcriptome analysis reveals the effect of LZ-8 on CD4+ T cells and CD8+ T cells [RNASeq3]

LZ-8 is a dimeric protein isolated from Ganoderma lucidum. It has immunomodulatory activity on T cells. In order to fully analyze the immunomodulatory effect of LZ-8 on CD4 + T cells and CD8 + T cells, we first sequenced the transcriptome of mouse T lymphocytes treated with LZ-8 for 15 hours, then compared the differential genes regulated by LZ-8 with those activated in CD4 + T cells and CD8 + T cells in GSE126116 data, and finally obtained the core biological process and signal pathway induced by LZ-8 to regulating the activation state of CD4 + T cells and CD8 + T cells. Overall design: mRNA profiles of T lymphocytes treated by LZ-8 for 15h were generated by deep sequencing, in triplicate, using Ion PITM Chip.