Sensitivity of MiaPaCa2 cells to E2F inhibitor Ly101-4B.

MiaPaCa2 cells where screened with a set of original compounds with anticancer activity available at our laboratory. From these compounds we identified the nucleoside analogue ly101-4B as a strong inhibitor of the E2F activity. We confirmed its activity by studying its effect on MiaPaCa2's cell viability , caspase 3/7 activity and LDH release, to measure cell growth, apoptosis and necrosis after treatment with LY101-4B 25 µM. Then, to investigate the mechanism of action of ly101-4B we performed a transcriptomic analysis by using an Affymetrix approach (Genechip® Human Gene 1.0 ST Arrays). MiaPaCa2 cells were analyzed after vehicle or LY101-4B 50 µM treatment for 24 h. Two control and two treated biological replicates were analyzed.