Expression data from livers of 6 and 10-weeks garlic-fed mice

Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in control (cellulose) and garlic-fed C57BL/6J mice serendipitously infected with Pasteurella multocida and identified acute phase response genes, particularly Lcn2 and Orm2, as the major players in the innate response. Also dieraty garlic suppressed pasteurella infection in C57BL/6J mice. 8-week old male C57BL/6J mice were divided into two diet groups (two cages of each diet) and maintained at a non-barrier facility. Mice were fed (ad libitum) a special diet (Yeh and Yeh, 1994) with 4% cellulose (Control group, 3 mice) or 4% lyophilized raw garlic powder (Treatment group, 3 mice). At the end of the 6 and 10-week treatment, liver organs were used for RNA isolation and arraying.