Self-cleaving guide RNAs enable pharmacological selection of precise gene editing events in vivo
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https://www.ncbi.nlm.nih.gov/sra/SRP404483
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In this study we compared the gene expression of mice that had received the rAAV-GR-Cypor scgRNA and rAAV-spCas9 with and without APAP selection. APAP selected mice were also compared to mice which had received the rAAV-GR-Hpd miRNA. Overall design: Livers from terminally harvested mice were isolated and RNA was extracted using RNAzol. Gene expressions profiles and expression of integrated rAAV constructs were compared between the different groups.
创建时间：
2023-01-04
