Comparison of sRNA populations obtained using HD or standard Illumina adapters in M truncatula, without TAP treatment

The sRNA population (sequence identity and distribution of abundances) in M truncatula was assessed using High Definition (HD) [Sorefan et al 2012, Xu et al 2015] or standard Illumina adapters; the usage of HD adapters revealed a more diverse population of sRNAs (in particular, it revealed the presence of more unique 24mers) Overall design: sRNA libraries from M truncatula leafs were created using HD and standard Illumina adapters, without a TAP treatment; the resulting libraries were compared side by side.