Suppression of cytoplasmic incompatibility in the leaf-mining fly Liriomyza sativae with a nuclear Wolbachia insert

Cytoplasmic incompatibility (CI) drives maternally transmitted endosymbionts such as Wolbachia through insect populations by inducing embryonic mortality when infected males fertilize uninfected females. CI is controlled by Wolbachia cif operons that are categorized into multiple phylogenetic Types. CI strength is further shaped by poorly understood host factors, including development and genetic background. To study the strength of CI across different host species, we genotyped a Japanese field population of Liriomyza sativae. By uncovering paternal transmission of Wolbachia genic elements, we collected strong evidence of horizontal genome transfer, including Type I and Type V cif operons, from Wolbachia into the nuclear genome of L. sativae. We established a transinfection of wLtri in L. sativae, a Wolbachia variant that induces strong CI in Liriomyza trifolii. No CI was observed in both intraspecific and interspecific reciprocal crosses with L. trifolii, suggesting that both uninfect..., crossing_experiment_results.csv:
The dataset was collected through controlled crossing experiments involving four different strains of Liriomyza trifolii and Liriomyza sativae with varying Wolbachia infection statuses. Below is a breakdown of the data collection and processing methods:
1. Experimental Design & Data CollectionStrains Used:L. trifolii (Wolbachia-infected and antibiotic-treated uninfected strains)L. sativae (Wolbachia-transinfected and uninfected strains)
Crossing Design:A full factorial crossing experiment was performed, yielding 16 different combinations.Individual Selection & Handling:Only unmated individuals were used, ensuring standardization of reproductive conditions.L. trifolii uninfected individuals were used from the third generation after antibiotic treatment to avoid direct antibiotic effects.L. sativae infected individuals were used from the third generation after Wolbachia transinfection via microinjection.Mating Conditions:1 female + 2 males per test..., , # Suppression of cytoplasmic incompatibility in the leaf-mining fly *Liriomyza sativae* with a nuclear *Wolbachia* insert

[https://doi.org/10.5061/dryad.41ns1rnrg](https://doi.org/10.5061/dryad.41ns1rnrg)

## Description of the data and file structure

This dataset contains reproductive and *Wolbachia* transmission data from crossing experiments between infected and uninfected strains of *Liriomyza trifolii* and *Liriomyza sativae*. It includes egg counts, hatching rates, emergence rates, sex ratios, and *Wolbachia* infection rates.

Additionally, the dataset comprises nucleotide sequences of *fbpA*, *cifA*, *cifB*, *clpA*, and *pyrD*, obtained through PCR amplification and Sanger sequencing. These data were collected to investigate the genetic composition and functional characteristics of *Wolbachia* genes, with a focus on horizontal gene transfer and cytoplasmic incompatibility mechanisms. This dataset provides insights into *Wolbachia* -host interactions and their evolutionary impli...,