Zebrafish Embryonic Heads Single-cell Sequencing

Processed single cell RNA-sequencing data for 2 samples: Cells from the cranial region of approximately 50 zebrafish embryos each at time points 24 hours post-fertilization (hpf) and 28 hpf. Sequencing was done with 10X Genomics Chromium.

Data are organized into 2 corresponding folders. Each folder contains HDF5 format (.h5) files with barcode matrices for the raw features (raw_feature_bc_matrix.h5) and filtered features (filtered_feature_bc_matrix.h5). Another H5 file (molecule_info.h5) contains per-molecule information for all molecules that contain a valid barcode, a valid UMI, and were assigned with high confidence to a gene or Feature Barcode. An Excel spreadsheet and HTML file summarize all the metrics for that particular sequencing dataset. A .cloupe file can be imported into the 10X genomics Loupe R package to analyze the Seurat Objects for data visualization and exploration.

本数据集包含了经过处理的单细胞RNA测序数据，共涉及2个样本：分别来自大约50尾斑马鱼胚胎头颅区域的细胞，在受精后24小时（hpf）和28小时（hpf）的时间点进行测序。测序采用10X Genomics Chromium平台进行。数据被组织成2个相应的文件夹，每个文件夹包含HDF5格式（.h5）的文件，其中包含原始特征（raw_feature_bc_matrix.h5）和过滤特征（filtered_feature_bc_matrix.h5）的条形码矩阵。另一个H5文件（molecule_info.h5）包含了所有具有有效条形码、有效UMI，且被高置信度分配至基因或特征条形码的分子信息。一个Excel电子表格和HTML文件总结了该特定测序数据集的所有指标。.cloupe文件可导入10X genomics Loupe R软件包中，用于Seurat对象的可视化分析和探索。