Overexpression lethality of lam cDNA constructs

The lethality of overexpressing lam cDNA ubiquitously or in mesoderm, but not in hemocytes or neurons. The GAL4 drivers used were da - daughterless, strong ubiquitous expression; arm - armadillo, weaker ubiquitous expression, P{GawB}how24B- mesoderm; P{GawB}C23 - transverse muscles; hemese - hemocytes [49]; and nrv2 - nervana, nervous system. Expression was driven by the indicated GAL4 drivers, and cultures were held at 25��C and 29��C. Viability was scored as the survival of any adults from ��70 embryos. Note that ubiquitous lam overexpression was lethal in every case except for the presumably weaker expressed cDNA construct driven by the weak arm ubiquitous GAL4 driver at the lower temperature (less GAL4 activity).