Transcriptomic analysis of liver lymphatic endothelial cell (LyEC) isolated from sham or bile duct ligation (BDL)-induced cholestasis mouse model

This experiment aims to determine the transcriptomic alteration in liver lympahtic endothelial cell (LyEC) in response to bile duct ligation (BDL)-induced cholestatic injury, which known to produce severe form of fibrosis and cirrhosis. We uncover a transcriptomic changes in LyEC associated with early-stage fibrosis/cirrhosis and late-stage decompensated liver cirrhosis. This work determines the functional change in lymphatic vessels and provides background necessary to discover therapeutic targets for improving LyEC function in the progression of liver diseases. For the transcriptomic profile, liver lymphatic endothelial cells (LyECs) were isolated from LyEC-specific GFP reporter mice (IL7GFP/knock-in) liver at 2 weeks (n=3) and 4 weeks (n=3) after bile duct ligation (BDL) surgery or sham (n=3) mice.