Comparing the transcriptome of cultured miR-206 KO FAPs and wildtype (WT) FAPs

FAPs isolated by FACS from miR-206 KO mice and WT mice were grown in culure and then placed in basal medium (BM) for 48 hours to assess the transcriptomic changes FAPs were isolated from miR-206 KO and WT mice by FACS and grown in culture with growth medium (GM) until ~90% confliuent. The cells were then switched to BM and maintained for 48 hours, at which time total RNA was isolated. This RNA was delivered to the Stanford Functional Genomics Center for library preparation and sequencing. Two replicates per condition were completed.