Metal-Dependent Hydrolase 1 Encoded by “Candidatus Liberibacter Asiaticus” Interferes with Citrus Immunity by Targeting Papain-like Cysteine Proteases

Citrus Huanglongbing (HLB), caused
by “Candidatus Liberibacter
asiaticus” (CLas), is a devastating citrus disease
worldwide, and its pathogenesis remains poorly understood. Here, we
investigated the virulence function of a Sec-dependent effector, metal-dependent
hydrolase 1 (MDH1), which is significantly upregulated in citrus during
CLas infection compared to in psyllids. Overexpression of MDH1 in citrange reduced its resistance to Xanthomonas citri subsp. citri.
RNA-seq and RT-qPCR analyses revealed that MDH1 reprogramed
the transcription of genes involved in plant–pathogen interactions
and suppressed the expression of defense-related genes. Crucially,
MDH1 interacted with the Citrus sinensis aleurain-like protease (ALP), a papain-like cysteine protease (PLCP),
specifically with the cysteine protease domain. MDH1 also interacted
with other PLCP family members and significantly inhibited the expression
of several PLCP family genes. This study uncovers how MDH1 suppresses
plant immunity by interacting with citrus PLCPs, providing useful
information for designing targeted inhibitors to combat HLB.