Chromatin accessibility analysis of adipose progenitors (AP).

We hypothesized that core transcriptional regulatory circuitries analysis could be used in conjunction with gene expression profiling data to nominate specific TFs for additional functional studies of AP cell state. To accomplish this analysis, we utilized the assay of transposase-accessible chromatin coupled to massively parallel sequencing (ATAC-seq) to identify putative enhancers in APs. The AP preparation involved adipose tissue digestion, and negative selection of the stromal vascular fraction (depletion of CD31+ endothelial cells and Lineage positive cells). 4 samples were anlyzed. 2 groups of adipose progenitors were isolated from subcutaneou(SAT) and visceral (VAT) adipose tissue from 16 wks C57BL/6 mice, in duplicate.