An integrated toolkit for human microglia functional genomics

Microglia, the brain’s resident immune cells, play vital roles in brain development, and disorders like Alzheimer’s disease (AD). Human iPSC-derived microglia (iMG) provide a promising model to study these processes. However, existing iMG generation protocols face challenges, such as prolonged differentiation time, lack of detailed characterization, and limited gene function investigation via CRISPR-Cas9. In this study will generated iMG from human iPSCs using our newly developed protocol. We used single cell RNA-Seq to study the transcriptomic profiles of our newly formed iMG. The single cell RNA-Seq data under this accession is from wild type iMG (Hashtag 7) and OTHER KO iMG (Hashtag 8) iPSC-derived microglia (iMG) were harvested from in vitro cultures, washed with PBS and hashtaged with hash tagging antibodies as: WT: Hashtag 7, OTHER KO Hashtag 8. scRNA-Seq was performed using 10X Genomics Chromium Next GEM single-cell 3' Reagent kit v3.1 leveraging its Feature Barcode technology.