DEVELOPMENT OF A COMPREHENSIVE APPROACH FOR THE CHARACTERIZATION OF POLAR PLANT DECOCTIONS BY ORTHOGONAL LC-MS REVERSE PHASE, HILIC, AND NMR METABOLITE PROFILING: THE CASE OF COMBRETUM MICRANTHUM

Publication which aimed to comprehensively characterize the metabolite profile of C. micranthum decoction prepared following traditional practices. To maximize coverage of both polar and non-polar metabolites, we adopted a dual chromatographic approach combining HILIC and RP chromatography. Metabolite annotation was performed using UHPLC-HRMS/MS, with the major polar constituents detected by charged aerosol detection or evaporative light scattering detector (CAD/ELSD) and quantified by quantitative ¹H-NMR spectroscopy. In addition, HILIC was used for the purification of polar metabolites through a dry-loading strategy that allows introduction of poorly soluble compounds into solvent systems containing high levels of organic phase.