Biallelic TET2 mutation sensitizes to 5'-azacitidine in acute myeloid leukemia. Stolzel et al. JCI Insight. [RNA-Seq]

To investigate the impact of TET2 loss on global transcription in HEL acute myeloid leukaemia (AML) cells. HEL AML cells, which have a monoallelic TET2 mutation, were targeted by CRISPR to inactivate the remaining TET2 allele, generating isogenic cell clones with either monoallelic or biallelic TET2 mutation. Overall design: We then performed comparative gene expression profiling analysis using data obtained from RNA-seq of 3 indepenent cell clones with monoallelic TET2 mutation and 3 independent cell clones with biallelic TET2 mutation.