notho_tanzania_ddRAD_10occup.vcf

Data was obtain by double-digest restriction site associated DNA (ddRAD) sequencing. The quality and quantity of raw sequencing reads were checked using FastQC (Andrews, 2010) and MultiQC (Ewels et al., 2016) before and after data preparation. The program Skewer (Jiang et al., 2014) was used to demultiplex samples and remove restriction enzyme sites, inline barcodes and adapters. The data was assembled by mapping onto the reference genome of <i>Nothobranchius furzeri </i>(Genbank: GCA_001465895.2) and SNPs were extracted using iPyrad software 0.9.61 (Eaton and Overcast, 2020). The .vcf file contains the dataset with 10% occupancy across the dataset of 237 individuals of genus <i>Nothobranchius</i> from Tanzania and one specimen of <i>Fundulosoma thierryi</i> (used as an outgroup).