Many bacterial pathogens produce extracellular proteases that are involved in the degradation of the host extracellular matrix. Dichelobacter nodosus, which causes ovine footrot, is one such pathogen,

NIAID Data Ecosystem40

Quantitative profiling of protease specificity

蛋白酶底物特异性

酶学定量分析

Proteases comprise an important class of enzymes, whose activity is central to many physiologic and pathologic processes. Detailed knowledge of protease specificity is key to understanding their funct

DataONE2021-02-03 更新30

Kinetic parameters of the purified wild-type and mutant SAPB enzymes with selected synthetic peptide substrates.

动力学分析

蛋白酶底物特异性

Assays were performed using the purified proteases in 100 mM buffer containing 2 mM Ca2+, and 0.2 mM to 50 mM synthetic peptide substrates (YLV and FAAF) at suitable pH. The samples were incubated for

NIAID Data Ecosystem50

Profiling active site specificity for proteases ADAMTS13 and Thrombin. Inovirus M13

蛋白酶底物特异性

噬菌体展示高通量测序

This project involves high throughput sequencing of phage display libraries to profile active site specificity of proteases ADAMTS13 and Thrombin

NIAID Data Ecosystem30