Deep-sequencing profile of the distinguishing nucleotides between CPV-2a (KM457143) and 364-rec recombinant (KM457139) strains in sample 364.

a The left fragment (NS gene) of the 364-rec strain was derived from a CPV-2c strain, and the right fragment was from a CPV-2a strain. b Coverage: number of times a nucleotide is read during the sequencing process. c The recombination breakpoint in the 364-rec recombinant strain falls in a 117-nt region between two constant parental markers (positions 2433 and 2549). d The difference in the 2907 position corresponds to a single-nucleotide polymorphism in the 2a homologous region of the co-infecting strains. Deep-sequencing profile of the distinguishing nucleotides between CPV-2a (KM457143) and 364-rec recombinant (KM457139) strains in sample 364.