Gene expression of PC3ML, metastatic prostate cancer cells, with or without HAMVEC EV C, HAMVEC EV PIC, or ATEV treatment and/or expressing varying levels of endogenous TWIST1

The aim of this study was to explore the potential link between inflammation-induced endothelial dysfunction and prostate cancer phenotypic plasticity in vitro, and to explore the role of endogenous Twist1 In this study PC3ML expressing either wild type, deficient or surplus levels of Twist1 were either untreated or treated with extracellular vesicles (EV)derived from control or proinflammatory-cytikine treated human adipose microvascular endothelial cells or from EV collected from adipose tissue of subjects undergoing bariatric surgery. RNA was then isolated from the cells and gene expression assessed using the NanoString Pan Cancer Progression panel.