depolymerase. Phage and depolymerase

In Iran, Klebsiella pneumoniae is one of the dangerous bacteria in nosocomial infections. In Klebsiella pneumonia, antibiotic resistance is very high and with the production of ESBL, it is resistant to almost all common antibiotics. In our project, we have tried to isolate and replicate specific phages against this bacterium so that we can use them in next phage therapy projects. In this project, sampling was performed of the wound from a 62-year-old patient who had a sever bedsore. Then, after passing the sewage of Bu-Ali Hospital in Qazvin through a 0.22 μm filter, was added Klebsiella pneumoniae, , and the desired phage was able to destroy Klebsiella pneumoniae. After using plaque assay technique and purification, we tacked electron microscopic photo. The phage is belong to siphoviridae family with dimensions (Capsid=60 nm, Tail=130nm).