Effect of UBLCP1 knockdown on gene expression in HeLa S3 cells

Human UBLCP1 is a unique nuclear phosphatase which has both ubiquitin-like domain and CTD phosphatase-like domain. UBLCP1 interacts with proteasome subunit Rpn1 and suppresses the activity of proteasome, but its role in gene expression is unknown. To identify genes whose expression levels are affected by UBLCP1 knockdown in human cultured cells, we performed gene expression profiling by microarray analysis. HeLa S3 cells were seeded on 6-well plates at 1.0 × 10^5 cell/well and cultured at 37°C 24 h before transfection. Cells were transfected with either negative control siRNA or siRNA specific to human UBLCP1 gene using Lipofectamine RNAi MAX (ThermoFisher) and further cultured for 72 h at 37°C.