Comparison of the response of Col-0 and ate1 ate2 N-degron mutant to flg22

Arabidopsis mutants of the N-degron pathway have been shown to be either susceptible or resistant to a wide range of pathogens. This includes mutants for the arginine-transferases (ate1-2 ate2-1) and for the downstream E3 ubiquitin ligase PRT6 (prt6). In addition, ate1-2 ate2-1 mutants have been shown to have dampened gene expression changes upon inoculation with Pseudomonas syringae AvrRpm1. Here, we tested whether ate1-2 ate2-1 mutants were impaired for pattern-triggered immunity (PTI) and determined gene expression changes of wild-type Col-0 and of ate1-2 ate2-1 mutant seedlings in the absence/pressence of flg22. Overall design: To investigate transcriptional differences between 10-day old seedlings of Col-0 (wild type) and the ate1-2 ate2-1 N-degron mutant upon 1 hour treatment with 1 uM flg22 (or mock control). 3 biological replicates per sample.