Single nuclear RNASeq Analysis of Anaplastic and Favourable Histology Hu_Wilms Tumor

Wilms tumor is the most common pediatric kidney cancer. The best predictor of clinical outcome for Wilms tumor patients is how their tumor looks under the microscope (histology). Usually, the resistant/anaplastic component of the tumor makes up only a fraction of the total number of cells in the cancer. A critical barrier to understanding therapeutic resistance in this disease is that studies performed to sequence the resistant component is diluted by the other components of the tumor.Therefore, the current proposal aims to using single-nuclear-RNA sequencing to isolate the gene expression patterns of individual cell types in Wilms tumor and to focus on the anaplastic/resistant cells. Patient frozen tumor was analyzed using single cell nuclear RNASeq. The single cell nucleus was isolated and Single nuclear RNAseq was performed using 10X genomics -Single cell gene expression kit V3.