Quantification of antibody–drug conjugate PYX-201 in rat and monkey plasma via ELISA and its application in preclinical studies: Supplementary data

Aim: PYX-201 is a novel antibody–drug conjugate targeting the extra domain B splice variant of

fibronectin in the tumor microenvironment. Accurate quantification of PYX-201 is critical for PYX-

201 pharmacokinetics profiling in preclinical studies. Materials & methods: ELISA was performed using

reference standard PYX-201, mouse monoclonal anti-monomethyl auristatin E antibody, mouse IgG1,

mouse monoclonal anti-human IgG horseradish peroxidase and donkey anti-human IgG horseradish

peroxidase. Results: This assay was validated at 50.0–10,000 ng/ml in rat dipotassium EDTA plasma and

250–10,000 ng/ml in monkey dipotassium EDTA plasma. Conclusion: This is the first time for a PYX-201

bioanalytical assay in any matrix to be reported.