USPA's application to cell puncture

This figures show a sequence of photos of puncturing into the zerbrafish embryo. Initially, the tip approached the embryo orthogonally to the slot and punctured into the embryo at the instant that it was in contact with the cell membrane. Subsequently, the USPA caused the tip to retract from the embryo; this process changed the embryo’s position possibly because of the dragging force between the pipette and the membrane [48]. Followingly, the USPA moved in the lateral direction to the next embryo, and finally, it completed the process. These results demonstrate the USPA’s potential to cell puncture, benefiting from the untethered two-DOF movements.