Data from: Development and Validation of KASP Markers for Wheat Streak Mosaic Virus Resistance Gene Wsm2

Wheat streak mosaic virus (WSMV) can cause significant yield loss in wheat (Triticum aestivum L.) in the Great Plains of North America. A recently identified WSMV resistance gene, Wsm2, was mapped to chromosome 3BS in germplasm line 'CO960293–2'. Effective genetic markers tightly linked to the gene will enhance the selection of WSMV-resistant lines through marker-assisted selection. We have mapped Wsm2 using a high-density map developed from the wheat 90K Infinium iSelect single-nucleotide polymorphism (SNP) array with recombinant inbred lines from the cross between CO960293–2 and susceptible cultivar 'TAM 111'. Array-based SNPs that mapped within 4 cM of Wsm2 on chromosome 3BS were converted to Kompetitive Allele Specific Polymerase Chain Reaction (KASP) assays in this study. Six KASP SNPs were validated in two doubled haploid populations developed from crosses of 'RonL' × 'Ripper' and 'Snowmass' × 'Antero'. RonL and Snowmass possess the Wsm2 gene from CO960293–2. Three closely linked KASP SNPs, converted from IAAV6442, BS00018764_51, and wsnp_Ra_c16264_24873670, showed high sensitivity and specificity (0.83 ≤ sensitivity ≤ 0.97, 0.89 ≤ specificity ≤ 0.99). The latter two were also validated in six F2 breeding populations. These three KASP SNPs were effective in differentiating resistant and susceptible genotypes. Comparative mapping was performed using sequences of SNPs flanking Wsm2 and identified candidate genes and regions in Brachypodium and rice (Oryza sativa L. ssp. japonica). The KASP SNPs developed in this study should be useful for marker-assisted selection of Wsm2 in wheat breeding programs, and the newly constructed map will also facilitate map based cloning of Wsm2. Resources in this dataset:Resource Title: Supplemental Table S1: A list of candidate genes identified in the region between wheat markers GENE-1856_1005 and Tdurum_contig57914_1144 2 to Brachypodium and rice orthologoues - Download PDF. File Name: csc2cropsci2016040234-sup-0001.pdfResource Description: Brachypodium and rice candidates for Wsm2 orthologous to the colinear region in wheat chromosome 3B that were identified are presented.

小麦条斑花叶病毒（Wheat streak mosaic virus，WSMV）可导致北美大平原地区小麦（Triticum aestivum L.）产量显著损失。近期鉴定出的WSMV抗性基因Wsm2，已被定位到种质资源系'CO960293–2'的第3BS染色体上。与该基因紧密连锁的遗传标记能够有效提升通过标记辅助选择对WSMV抗性品种的筛选。本研究利用由小麦90K Infinium iSelect单核苷酸多态性（SNP）微阵列构建的高密度图谱，结合CO960293–2与感病品种'TAM 111'的重组自交系，对Wsm2进行了定位。本研究将位于第3BS染色体上Wsm2附近4 cM范围内的SNP转换为Kompetitive Allele Specific Polymerase Chain Reaction（KASP）检测。六个KASP SNPs在由'RonL' × 'Ripper'和'Snowmass' × 'Antero'杂交产生的双倍体纯合群体中得到了验证。RonL和Snowmass均含有来自CO960293–2的Wsm2基因。三个与Wsm2紧密连锁的KASP SNPs，分别来自IAAV6442、BS00018764_51和wsnp_Ra_c16264_24873670，表现出高灵敏度（0.83 ≤ 灵敏度 ≤ 0.97）和高特异性（0.89 ≤ 特异性 ≤ 0.99）。后两者亦在六个F2育种群体中得到了验证。这三个KASP SNPs在区分抗性和感病基因型方面表现有效。通过Wsm2两侧SNP序列的比较作图，识别了拟南芥和水稻（Oryza sativa L. ssp. japonica）中的候选基因和区域。本研究开发的KASP SNPs有望用于小麦育种中对Wsm2的标记辅助选择，而新构建的图谱也将有助于Wsm2的基于图谱的克隆。数据集资源：资源标题：补充表S1：位于小麦标记GENE-1856_1005和Tdurum_contig57914_1144 2之间的区域中鉴定出的候选基因列表对应拟南芥和水稻同源基因 - 下载PDF。文件名：csc2cropsci2016040234-sup-0001.pdf资源描述：展示了与小麦3B染色体上线性区域同源的Wsm2同源基因在拟南芥和水稻中的候选基因。