Transcriptomic analysis of KDM4B-mediated gene in C4-2B prostate cancer cells. Transcriptomic analysis of KDM4B-mediated gene in C4-2B prostate cancer cells

Histone lysine demethylase KDM4B is often overexpressed in prostate cancer and links to poor survival outcome. Recent evidence suggests that KDM4B serves as a potential therapeutic target. The global transcriptomic analysis between control and KDM4B-knockdown C4-2B cells reveals the metabolic genes involved in Warburg effect are downregulated after knocking down the expression of KDM4B, indicating its role in tumor growth. Overall design: KDM4B control and knockdown cell lines were established in C4-2B cells based on the lentivirus system: LKO (control) and shKDM4B (shRNA-specific RNA) lines. Global expression analysis was then performed (pLKO vs. KDM4B-knockdown). Microarray analysis was conducted by the National Health Research Institutes, using Affymetrix GeneChip Human Gene 2.0 ST Array (Affymetrix, Santa Clara, California, USA).