The role of three heat shock protein genes in the immune response to Aeromonas hydrophila challenge in marbled eel, Anguilla marmorata

Real-time fluorescent quantitative-PCR was used to evaluate tissue-specific distribution and mRNA expression levels of three Amhsps subjected to the infection with Aeromonas hydrophila. The mRNA expression three Amhsps in eight tested tissues, including liver, heart, muscle, gill, spleen, kidney, brain and intestine tissues of A. marmorata was evaluated to reveal the major expression distribution in liver, intestine, muscle and heart. After pathogen challenge treatments, mRNA transcriptions of three Amhsps revealed a significant regulation at various time points in the same tissue.The expression level of Amhsps was calculated by 2⁻^ΔΔ^C^T method and subjected to statistical analysis.