A2PP has minimal effects on EGFR-ANXA2 signaling, migration of gastric cancer cells, or the localization of ANXA2.

(A) Western blotting of phospho-EGFR, phospho-ANXA2, EGFR, and ANXA2 from AGS and BGC823 cells treated with A2PP for 24 hr. GAPDH was used as loading control. (B) Representative images of migration of AGS and BGC823 cells treated with A2PP for 24 hr. Scale bars, 200 μm. (C) Statistical summary of migration assay. Mean ± SD from three experiments with triplicate samples. ns, no significance. (D) Immunofluorescence of ANXA2 localization (red) in AGS and BGC823 cells treated with A2PP for 24 hr. Scale bars, 5 μm.