Silencing miR-31 in naive CD4+ T cells by antagomiRNA

To describe the global profile of genes regulated by miR-31, we compared the transcriptomics between miR-31 silenced and antagomir control treated naive CD4+ T cells. Interestingly, the cells could be separated into two subsets according FCS and SSC by flow cytometer after the silence of miR-31, one (~65%) was small sized cells with low granularity, the other (~30%) was large cells with high granularity, whereas the antagoNC treated naïve CD4+ T cells only contain a marginal fraction of large cells. We pooled “antagoNC”, “antagomiR31S” and “antagomiR31L” naïve CD4+ T cells from 5 healthy donors, respectively, and analyzed the differential genes among those three subsets. naive CD4+ T cells were transfected with antagomiR31 and antagoNC and cultured for 48 hours. Cells were sorted by flow cytometer according to small size and granularity. Sample “antagoNC”, “antagomiR31S” and “antagomiR31L” naïve CD4+ T cells were pooled from 5 healthy donors, respectively.