Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine

The purpose of this study was to elucidate potential effects of fxr mutation on the functional specification of intestinal epithelial cells. To do this, we sorted GFP-positive cells from TgBAC(cldn15la:GFP) transgenics of either fxr+/+ or fxr-/- fish larvae and subjected them to 10X Genomics single-cell RNA-seq. Our results uncovered the requirement of fxr in gene expression and differentiation among multiple intestinal epithelial cell types. Analysis of sorted cells from 6 days post-fertilization zebrafish larvae that were either fxr+/+ (WT) or fxr-/- (MUT)