Transcriptional response of human duodenal enteroid monolayers to norovirus infection

The goal of this study was to evaluate the transcriptional response of 4 human duodenal enteroid lines on monolayers to norovirus infections (GII.4). Enteroids were plated as monolayers in Intesticult (Stem Cell Technologies) proliferation medium. After 1 day of cell growth as a monolayer, the proliferation medium was changed with differentiation medium for 5 days. Five-day-differentiated monolayers were washed and were either mock-infected or inoculated with human norovirus supplemented with 500?µM glycochenodeoxycholic acid (GCDCA), for 1 to 2?h at 37°C. Total RNA was extracted using the Qiagen RNeasy kit and paired-end Illumina sequencing was performed. Overall design: A total of 8 mRNA profiles were generated by paired-end sequencing. The duodenal enteroid lines used were D4, D8, D104, and D109. Each of these cell lines were plated as monolayers on collagen, differentiated and mock-infected or inoculated with human norovirus supplemented with 500?µM GCDCA.