Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Expression and purification of biotinylated full length (FL) USP51-835 and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5171-290 constructs for the development of a displacement assay.