In-vitro CCM tube formation

This set includes segmented images of the control cell (wild-type HUVEC) and cells with knockdown (KD) of the three Cerebral Cavernous Malformation (CCM) proteins, CCM1 (or KRIT1), CCM2, and CCM3 (or PDCD10), which disrupts the integrity of multicellular mesh. In addition, the control and KD cells were treated with an inhibitor of Rho-associated protein kinase (ROCK), which was shown to be over-activated in CCM KD cultures (Chernaya O, Zhurikhina A, Hladyshau S, Pilcher W, Young KM, Ortner J, Andra V, Sulchek TA, Tsygankov D. 2018. Biomechanics of Endothelial Tubule Formation Differentially Modulated by Cerebral Cavernous Malformation Proteins. iScience 9:347-358.8)

本数据集包含控制细胞（野生型HUVEC）以及敲低三种脑静脉畸形（CCM）蛋白（CCM1或KRIT1）、CCM2和CCM3（或PDCD10）的细胞图像，这些蛋白的敲低破坏了多细胞网状结构的完整性。此外，控制细胞和敲低细胞均经Rho相关蛋白激酶（ROCK）抑制剂处理，该抑制剂在CCM敲低细胞培养中被证实过度激活（Chernaya O, Zhurikhina A, Hladyshau S, Pilcher W, Young KM, Ortner J, Andra V, Sulchek TA, Tsygankov D. 2018. 脑静脉畸形蛋白对内皮管状结构生物力学差异调节。iScience 9:347-358.8）。