Genetic deletion of RUNX1 or RUNX2 in adipocytes enhanced beige fat formation [ATAC-seq]

To uncover novel transcriptional regulatory mechanisms of brown adipogenesis, we performed Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) to map genome-wide chromatin accessibility during the early stages of immortalized primary brown preadipocytes differentiation.Intriguingly, we identified a significant enrichment of the RUNX transcription factor binding motif within brown preadipocyte-specific peaks, suggesting a previously unrecognized role of the RUNX family as a potential barrier to brown adipogenesis Overall design: Differentiation from brown pre-adipocytes toward mature brown adipocyte stage day 0 and day 1