Transcriptome analysis on mature, non aged seeds of the spring barley landraces Cebada Capa and L94 and L94 NILs by RNA sequencing.. Transcriptome analysis of barley seeds

Widespread natural variation in barley offers an opportunity to study seed longevity in an important temperate cereal crop. For example, the spring barley landraces Cebada Capa (Argentina) and L94 (Ethiopia) display long and short-term seed longevity, respectively. We have used this contrast to identify and characterize seed longevity genes in barley by RNA sequencing analysis of mature, non aged seeds of Near Isogenic Lines (NILs) with a L94 background but harboring introgressions from Cebada Capa in four putative QTLs on chromosomes 1H and 2H.