LONG NON-CODING RNAs ASSOCIATED WITH METABOLIC TRAITS IN HUMAN WHITE ADIPOSE TISSUE

The aim of this study was to identify novel long noncoding RNAs (lncRNAs) that are differentially expressed in the subcutaneous region either in obesity or insulin resistance. A number of novel lncRNAs were differentially expressed in the subcutaneous region either in obesity or insulin resistance. Two lncRNAs (termed here as adipocyte specific insulin resistance related lncRNAs (ADLNRIs), ADLNRI 1 and ADLNRI 2) were influenced by both conditions and enriched in adipocytes. Knockdown of either by antisense oligonucleotides in fat cellsadipocytes altered lipolysis and adiponectin secretion, respectively. The 80 women included in this study were selected from the extremes of insulin sensitivity as measured by homeostasis model assessment of IR (HOMAIR) among 220 obese women who participated in a clinical trial on the effect of bariatric surgery (NCT01785134 at www.clinicaltrials.gov). All sampling and measurements were performed before or during bariatric surgery (laparoscopic gastric bypass). Participants were investigated at 8 AM after an overnight fast. Insulin sensitivity was assessed by HOMAIR and was calculated from fasting measures of glucose and insulin. High HOMAIR values indicate IR. The 40 women with the highest HOMAIR values and the 40 women with the lowest values were selected for inclusion in the present study. Differential gene expression analysis was conducted at the gene level using Limma, selecting for genes with a false discovery rate of <0.05