Expression data from Arabidopsis thaliana (Col-0) exposed to Arsenic, Hypoxia and their combination

Plants often face combinatorial stresses in their natural environment. Here arsenic (As) toxicity was combined with hypoxia (Hpx) in the roots of Arabidopsis thaliana as it often occurs in nature. The present work aimed to explore the effects of single and combined hypoxia and As stress applied at realistic stress levels to hydroponically grown A. thaliana. Arsenic as well as hypoxic growth conditions generate a characteristic signaling pattern, a significant part of which is mediated by ROS. The current study utilized the microarray to determine the overlapping signalling pattern and changes in gene expression for the defined stress combinaton compared to individual stresses. Roots of Arabidopsis thaliana (Col-0; WT), grown hydroponically for 32d were exposed to either 250 µM As (AsV; Na2HAsO4) alone or in combination with hypoxic nutrient media and also hypoxia alone. The plant material was harvested after 7d of treatment and root samples were used for RNA extrcation and subsequent hybridization on Affymetrix microarray chips. Three completely independent biological replicates were analyzed for each treatment (C1, C2, C3: Control; As1, As2, As3: Arsenic; Hpx1, 3, 5: Hypoxia; Hpx2, 4, 6: Hypoxia + Arsenic)