Metagenomes can be considered as mixtures of viral, bacterial, and other eukaryotic DNA sequences. Mining viral sequences from metagenomes could shed insight into virus–host relationships and expand v
196 reconstructed viral genomes of variable coverage. The viral sequences were derived by mapping de-hosted reads to reference viral genomes and computing consensus sequences.
RNA-seq reads were obtained from Chikushino-Hagiwara field. Barley leaf samples were collected in 2018. Illumina short-reads were utilized for assembling viral RNA contigs.
Retroviral reverse transcriptases (RTs) frequently switch templates during DNA synthesis, which can result in mutations and recombination. The relative rates of in vivo RT template switching during RN
