PCR amplification and microarray detection of alphaviruses.
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https://figshare.com/articles/dataset/_PCR_amplification_and_microarray_detection_of_alphaviruses_/774108
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Viral RNA derived from cell culture or infected mosquitoes were PCR amplified using GSPs. Amplicons were analyzed using the ArboChip5.1 microarray. VEE, Venezuelan equine encephalitis; WEE, western equine encephalomyelitis; POS, positive PCR amplification; NEG, negative PCR amplification; yes, detected by microarray; no, not detected by microarray; n.t., not tested.
aViruses propagated in cell culture and identified by microarray to species unless otherwise noted.
bVirus-infected mosquito used for microarray evaluations, microarray detected at least one target for each virus.
cPCR amplification produced a weak visible band.
dArboChip5.1 does not include probes specific to the target.
eMicroarray detection with genus-level probes only.
fTarget was detected but could not be differentiated between BBKV, OCKV, and SINV.
gTarget was detected but could not be differentiated between ONNV and IOV.
创建时间：
2013-08-15
