PCR amplification and microarray detection of alphaviruses.

Viral RNA derived from cell culture or infected mosquitoes were PCR amplified using GSPs. Amplicons were analyzed using the ArboChip5.1 microarray. VEE, Venezuelan equine encephalitis; WEE, western equine encephalomyelitis; POS, positive PCR amplification; NEG, negative PCR amplification; yes, detected by microarray; no, not detected by microarray; n.t., not tested. aViruses propagated in cell culture and identified by microarray to species unless otherwise noted. bVirus-infected mosquito used for microarray evaluations, microarray detected at least one target for each virus. cPCR amplification produced a weak visible band. dArboChip5.1 does not include probes specific to the target. eMicroarray detection with genus-level probes only. fTarget was detected but could not be differentiated between BBKV, OCKV, and SINV. gTarget was detected but could not be differentiated between ONNV and IOV.