Quantification of myoblast fusion after nocodazole and brefeldin A treatment.

Myoblast fusion index (the number of nuclei in myotubes divided by the total number of nuclei) was quantified in untreated and treated chick myogenic cultures. Cells were fixed with paraformaldehyde and double-labeled for desmin and DAPI. Nuclei were counted in fifty randomly chosen microscope fields (3 culture dishes for each of the experimental condition, 3 fields in each dish). Data are expressed as percentage of myoblast fusion (mean ± SEM). n.s. = not significantly different from control.*p