File S1 - S-Nitrosoglutathione Accelerates Recovery from 5-Fluorouracil-Induced Oral Mucositis

Figure S1, Real-time NO release profile of the 2.0 mM HPMC/GSNO formulation measured by chemiluminescence at 37°C. Inset: Integrated NO signal extracted from the kinetic curve, showing the total NO released from the formulation over the same time-scale. The straight lines show the linear regression of the experimental data and the calculated rates of NO release for two sections of the curve. Figure S2, The cheek pouches of animals subjected to 5-FU-induced oral mucositis showed a significant increase (P<0.05) of neutrophils on day 14 when compared with the Healthy and Mechanical Trauma (MT) control groups. 0.5 mM HPMC/GSNO treatment substantially (P<0.05) reduced the number of neutrophils in cheek pouch tissue compared with both the HPMC and Saline groups. Bars denote the means ± standard errors of the number of neutrophils from at six slides per group (6 animals per group). *denotes a significant difference (P<0.05) compared with the Healthy group; **denotes a significant difference (P<0.05) compared with the MT group, +denotes a significant difference (P<0.05) compared with the Saline group; #denotes a significant difference (P<0.05) compared with the HPMC group. Data were analyzed using the Kruskal Wallis and Mann Whitney tests.
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