Microbiome composition and microbial community structure in mosquito vectors Aedes aegypti and Aedes albopictus in northeastern Thailand, a dengue-endemic area.

This study characterized the microbiota associated with Aedes aegypti and Aedes albopictus larvae and subsequently emerged adults relative to the microbiota in water from their breeding sites in Thailand, a dengue-endemic area. The bacterial community changes from larval to adult stage possible transstadial transmission. Differential abundance analysis revealed that there were more bacterial genera associated with Ae. aegypti than with Ae. albopictus. Wolbachia was dominant and significantly higher frequencies in the Ae. albopictus adult male. The genus Blautia was particularly abundant in Ae. aegypti. Abundance of the genus Aquabacterium decreased from larva to adult in Ae. aegypti and Ae. albopictus. Adult Ae. aegypti females had greater proportions of Wolbachia, Blautia, Clostridioides, and Shinella than did males. The microbial community of Ae. albopictus larvae were dominated by the genus Serratia, while the genus Wolbachia was very abundant in female and male adults. The finding supports the development of Aedes mosquitos, which may include the likelihood of transstadial transmission acquired in the specific larval bacterial community.