Fecal microbiome analysis of CUMS and Ctrl mice at 0-week and 4-week

Fecal samples from animals screened from Ctrl or CUMS mice were used for microbiome analysis, including 8 control animals and 7 CUMS-induced depressive animals. To quantitatively assess differences in bacterial species composition between groups, shotgun metagenome sequencing was performed, and the data were analyzed using the Penn CHOP Microbiome Program. To identify communities within the microbiome, genomic DNA from each fecal sample was extracted using a QIAamp DNA stool Minikit according to the manufacturer instructions. The DNA was quantified using a spectrophotometer , and paired-end sequencing of the V3 to V4 region of the bacterial 16S rRNA was performed as previously described using an Illumina MiSeq system.