Fundulus heteroclitus isolate:FHET01 Genome sequencing and assembly. Fundulus heteroclitus isolate:FHET01

The Atlantic killifish (Fundulus heteroclitus) DNA was derived from a single male killifish (FHET 01). Sequences were generated on the Pacific Biosciences RSII instrument (V2 chemistry) to approximately 53x genome coverage based on a genome size estimate of 1.3 Gb. All SMRT sequences were assembled with the Canu algorithm then error corrected using the Arrow error-correction module. Additional polishing of the assembly for residual indels was done by aligning 50x coverage of Illumina data and the FreeBayes algorithm. Chromosomal level scaffold/contig files were generated by alignment to HiC data (Phase Genomics). For final chromosome assignments we aligned the scaffolds to the published genetic linkage map of Waits et al. Open Journal of Genetics, 6(01), 28. For questions regarding the use of the assembly please contact Dr. Wes Warren, Bond Life Sciences Center, University of Missouri, Columbia, MO or Dr. Andrew Whitehead, University of California, Davis, CA.Data use: The assembly sequence is made available to the community by Dr. Andrew Whitehead, University of California, Davis, CA with the following understanding: Users are free to analyze these data if the providers of this data are properly cited, any whole genome analyses should follow guidelines outlined in the Toronto agreement on the use of this assembly and any redistribution of the data should carry this notice.