scRNA-sequencing of in vitro cultured ILC2 from the whole brain tissue of 7 months old 3xTg-AD and Control mice.

ILC2 are potent producers of IL-5 and IL-13 and Th2 cytokines. We have found that 3xTg-AD mice exhibit diminished ILC2 numbers and functionality in the brain barriers. We have found through scRNA-seq that cultured brain ILC2 from 7 months old 3xTg-AD mice expressed significantly less IL-5 ane Areg but increased Gzma and Gzmb. We used the 10X Genomics platform to prepare scRNA-seq libraries to detail the global programme of different gene expression between cultured ILC2 in the whole brain tissue of 7 months old 3xTg-AD and Control mice.