Solubility and maximum yields of GPCRs produced using cell-free in vitro translation in the presence of Brij-35.

*Milligrams of receptor that could be produced in a 10 ml cell-free reaction. These yields were calculated from smaller batches of protein purified using immunoaffinity chromatography. Experiments showed that up to 1 mg/ml of protein could be produced, but that up to half could be lost during the purification process. The yields were determined by spectrophotometer readings.‡These yields were calculated by comparing the intensities of the receptor samples against a sample with a known concentration.