Small wedge MicroED data of Lysozyme, low-dose MTH1-8DG and high-dose MTH1-8DG

Small wedge MicroED data of Lysozyme, low-dose MTH1-8DG and high-dose MTH1-8DG.

A. Data information

Dataset 1: Lysozyme 

Dataset 2: MTH1 with ligand 8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE (PDB code: 8DG) collected using high fluence.

Dataset 3: MTH1 with ligand 8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE (PDB code: 8DG) collected using low fluence.

B. Data collection 

Data was collected with a CMOS CetaD detector installed on a Titan Krios G2 with an autoloader. 

Data collection software: EPUD

Cryo-EM infrastructure unit, Scilifelab, Stockholm.

C. Instrument and measurement settings

Instrumental:

Instrument

Titan Krios G3i

Radiation source

FEG

Accelerating voltage

300 kV

Wavelength

0.01968 Å

Probe Type

Nanoprobe

Beam Diameter

1.5 μm

Beam Convergence

Parallel beam

Detector

Ceta-D CMOS detector

Number of pixels in the image

1024 x 1024

Pixel size

5.6 µm x 5.6 µm

Effective Camera Length

3019.12 (lowdose), 3019.12 (highdose) and 1166 (lysozyme)

Hardware Binning

4

Experimental:

Data Type

Electron diffraction data - 3D ED, Microcrystal diffraction (MicroED)

Data collection method

Continuous rotation

Temperature (K) used during data collection

77 K

Number of crystals contributing to the data set

25 (lowdose), 20 (highdose), 61 (lysozyme)

Number of experimental frames

20 frame/data (lowdose), 20 frame/data (highdose), 30 frame/data (lysozyme)

tilt range, exposure time, tilt per frame

lowdose: 20 degree, 1s, 1 degree/frame

highdose: 20 degree, 1s, 1 degree/frame

lysozyme: 15 degree, 1s, 0.5 degree/frame

Software:

Software used for the data collection

EPUD

Software used for processing

AutoLEI with XDS

Authorship and Bibliography

Author(s) of the data

Lei Wang (ESR 10)

Related data

Publication(s)

Files and data formats

Image format

MRC