Average metabolic response in fertigated soils with control and residual vinasses as determined by Biolog EcoPlates

Vinasse is a by-product with a key role in the circular economy. In this work, we analyze sugarcane vinasse as culture medium for obtaining single and mixed inoculants. Trichoderma harzianum was cultured in single and sequential co-culture with Pseudomonas capeferrum or Rhizobium sp. Fungal biomass was higher in vinasse than in a laboratory medium. Residual vinasses presented almost neutral pH and lower conductivities and toxicity than raw vinasse. Fertigation with residual vinasses improves characteristics of soil evidenced in the total N, cation exchange capacity, urease and acid phosphatase, and the microbial metabolic diversity, in comparison to raw vinasse. The evaluation of the treatment indicates that vinasse is suitable for the production of inoculants containing T. harzianum and that the co-culture with P. capeferrum improves the characteristics of the residual vinasse in comparison to Rhizobium sp. Obtaining this valuable biomass in vinasse is relevant for the circular and green economy.

酒糟作为一种在循环经济中扮演关键角色的副产物，本研究将其作为获取单一及混合接种剂的培养基进行深入分析。通过单次及连续共培养假单胞菌（Pseudomonas capeferrum）或根瘤菌（Rhizobium sp.）的方式，培养木霉菌（Trichoderma harzianum）。相较于实验室培养基，酒糟中真菌生物量更高。剩余酒糟的pH值接近中性，其电导率和毒性均低于原酒糟。与原酒糟相比，使用剩余酒糟进行灌溉可改善土壤的特性，如总氮含量、阳离子交换能力、脲酶和酸性磷酸酶活性，以及微生物代谢多样性。对处理效果的评估表明，酒糟适用于生产含有木霉菌的接种剂，且与假单胞菌的共培养相较于根瘤菌，能显著提升剩余酒糟的特性。在循环经济和绿色经济中，从酒糟中获取这一宝贵生物量具有重要意义。